ZnO nanoflower-based photoelectrochemical DNAzyme sensor for the detection of Pb2+.
Identifieur interne : 000005 ( Main/Exploration ); précédent : 000004; suivant : 000006ZnO nanoflower-based photoelectrochemical DNAzyme sensor for the detection of Pb2+.
Auteurs : RBID : pubmed:24508815Abstract
Lead contamination is now widespread, and exposure to lead may cause adverse effects on human beings. In this study, a photoelectrochemical sensor based on flower-like ZnO nanostructures was developed for Pb(2+) detection, using a Pb(2+)-dependent DNAzyme as the recognition unit and a double-strand DNA intercalator, Ru(bpy)2(dppz)(2+) (bpy=2,2'-bipyridine, dppz=dipyrido[3,2-a:2',3'-c] phenazine) as the photoelectrochemical signal reporter. The ZnO nanoflower was fabricated on an indium tin oxide (ITO) electrode by the convenient hydrothermal decomposition method. The morphology and photoelectrochemical property of the ZnO nanoflowers were characterized by SEM, XRD and photocurrent measurements. DNAzyme-substrate duplex was assembled on an ITO/ZnO electrode through electrostatic adsorption. In the presence of Pb(2+), RNA-cleavage activity of the DNAzyme was activated and its substrate strand was cleaved, resulting in the release of Ru(bpy)2(dppz)(2+) from the DNA film and the concomitant photocurrent decrease. The detection principle was verified by fluorescence measurements. Under the optimized conditions, a linear relationship between photocurrent and Pb(2+) concentration was obtained over the range of 0.5-20 nM, with a detection limit of 0.1 nM. Interference from other common metal ions was found negligible. Applicability of the sensor was demonstrated by analyzing lead level in human serum and Pb(2+) spiked water samples. This facile and economical sensor system showed high sensitivity and selectivity, thus can be potentially applied for on-site monitoring of lead contaminant.
DOI: 10.1016/j.bios.2014.01.026
PubMed: 24508815
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">ZnO nanoflower-based photoelectrochemical DNAzyme sensor for the detection of Pb2+.</title><author><name sortKey="Zhang, Bintian" uniqKey="Zhang B">Bintian Zhang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Structures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China; Key Laboratory of Design and Assembly of Functional Nanostructures, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Structures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China; Key Laboratory of Design and Assembly of Functional Nanostructures, Chinese Academy of Sciences, Fuzhou, Fujian 350002</wicri:regionArea></affiliation></author><author><name sortKey="Lu, Lili" uniqKey="Lu L">Lili Lu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Optoelectronic Materials Chemistry and Physical Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Optoelectronic Materials Chemistry and Physical Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002</wicri:regionArea></affiliation></author><author><name sortKey="Hu, Qichang" uniqKey="Hu Q">Qichang Hu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Optoelectronic Materials Chemistry and Physical Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Optoelectronic Materials Chemistry and Physical Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002</wicri:regionArea></affiliation></author><author><name sortKey="Huang, Feng" uniqKey="Huang F">Feng Huang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Optoelectronic Materials Chemistry and Physical Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Optoelectronic Materials Chemistry and Physical Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002</wicri:regionArea></affiliation></author><author><name sortKey="Lin, Zhang" uniqKey="Lin Z">Zhang Lin</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Structures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China; Key Laboratory of Design and Assembly of Functional Nanostructures, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China. Electronic address: zlin@fjirsm.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Structures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China; Key Laboratory of Design and Assembly of Functional Nanostructures, Chinese Academy of Sciences, Fuzhou, Fujian 350002</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><date when="2014">2014</date><idno type="RBID">pubmed:24508815</idno><idno type="pmid">24508815</idno><idno type="doi">10.1016/j.bios.2014.01.026</idno><idno type="wicri:Area/Main/Corpus">000174</idno><idno type="wicri:Area/Main/Curation">000174</idno><idno type="wicri:Area/Main/Exploration">000005</idno></publicationStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lead contamination is now widespread, and exposure to lead may cause adverse effects on human beings. In this study, a photoelectrochemical sensor based on flower-like ZnO nanostructures was developed for Pb(2+) detection, using a Pb(2+)-dependent DNAzyme as the recognition unit and a double-strand DNA intercalator, Ru(bpy)2(dppz)(2+) (bpy=2,2'-bipyridine, dppz=dipyrido[3,2-a:2',3'-c] phenazine) as the photoelectrochemical signal reporter. The ZnO nanoflower was fabricated on an indium tin oxide (ITO) electrode by the convenient hydrothermal decomposition method. The morphology and photoelectrochemical property of the ZnO nanoflowers were characterized by SEM, XRD and photocurrent measurements. DNAzyme-substrate duplex was assembled on an ITO/ZnO electrode through electrostatic adsorption. In the presence of Pb(2+), RNA-cleavage activity of the DNAzyme was activated and its substrate strand was cleaved, resulting in the release of Ru(bpy)2(dppz)(2+) from the DNA film and the concomitant photocurrent decrease. The detection principle was verified by fluorescence measurements. Under the optimized conditions, a linear relationship between photocurrent and Pb(2+) concentration was obtained over the range of 0.5-20 nM, with a detection limit of 0.1 nM. Interference from other common metal ions was found negligible. Applicability of the sensor was demonstrated by analyzing lead level in human serum and Pb(2+) spiked water samples. This facile and economical sensor system showed high sensitivity and selectivity, thus can be potentially applied for on-site monitoring of lead contaminant.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="In-Process"><PMID Version="1">24508815</PMID><DateCreated><Year>2014</Year><Month>03</Month><Day>03</Day></DateCreated><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4235</ISSN><JournalIssue CitedMedium="Internet"><Volume>56</Volume><PubDate><Year>2014</Year><Month>Jun</Month><Day>15</Day></PubDate></JournalIssue><Title>Biosensors & bioelectronics</Title><ISOAbbreviation>Biosens Bioelectron</ISOAbbreviation></Journal><ArticleTitle>ZnO nanoflower-based photoelectrochemical DNAzyme sensor for the detection of Pb2+.</ArticleTitle><Pagination><MedlinePgn>243-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bios.2014.01.026</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0956-5663(14)00029-3</ELocationID><Abstract><AbstractText>Lead contamination is now widespread, and exposure to lead may cause adverse effects on human beings. In this study, a photoelectrochemical sensor based on flower-like ZnO nanostructures was developed for Pb(2+) detection, using a Pb(2+)-dependent DNAzyme as the recognition unit and a double-strand DNA intercalator, Ru(bpy)2(dppz)(2+) (bpy=2,2'-bipyridine, dppz=dipyrido[3,2-a:2',3'-c] phenazine) as the photoelectrochemical signal reporter. The ZnO nanoflower was fabricated on an indium tin oxide (ITO) electrode by the convenient hydrothermal decomposition method. The morphology and photoelectrochemical property of the ZnO nanoflowers were characterized by SEM, XRD and photocurrent measurements. DNAzyme-substrate duplex was assembled on an ITO/ZnO electrode through electrostatic adsorption. In the presence of Pb(2+), RNA-cleavage activity of the DNAzyme was activated and its substrate strand was cleaved, resulting in the release of Ru(bpy)2(dppz)(2+) from the DNA film and the concomitant photocurrent decrease. The detection principle was verified by fluorescence measurements. Under the optimized conditions, a linear relationship between photocurrent and Pb(2+) concentration was obtained over the range of 0.5-20 nM, with a detection limit of 0.1 nM. Interference from other common metal ions was found negligible. Applicability of the sensor was demonstrated by analyzing lead level in human serum and Pb(2+) spiked water samples. This facile and economical sensor system showed high sensitivity and selectivity, thus can be potentially applied for on-site monitoring of lead contaminant.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Bintian</ForeName><Initials>B</Initials><Affiliation>State Key Laboratory of Structures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China; Key Laboratory of Design and Assembly of Functional Nanostructures, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.</Affiliation></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Lili</ForeName><Initials>L</Initials><Affiliation>Key Laboratory of Optoelectronic Materials Chemistry and Physical Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.</Affiliation></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Qichang</ForeName><Initials>Q</Initials><Affiliation>Key Laboratory of Optoelectronic Materials Chemistry and Physical Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.</Affiliation></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Feng</ForeName><Initials>F</Initials><Affiliation>Key Laboratory of Optoelectronic Materials Chemistry and Physical Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.</Affiliation></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Zhang</ForeName><Initials>Z</Initials><Affiliation>State Key Laboratory of Structures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China; Key Laboratory of Design and Assembly of Functional Nanostructures, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China. Electronic address: zlin@fjirsm.ac.cn.</Affiliation></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>01</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biosens Bioelectron</MedlineTA><NlmUniqueID>9001289</NlmUniqueID><ISSNLinking>0956-5663</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">DNAzyme</Keyword><Keyword MajorTopicYN="N">Lead contamination</Keyword><Keyword MajorTopicYN="N">Photoelectrochemical sensor</Keyword><Keyword MajorTopicYN="N">ZnO nanoflower</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>10</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>1</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>1</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2014</Year><Month>1</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>2</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>2</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>2</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24508815</ArticleId><ArticleId IdType="pii">S0956-5663(14)00029-3</ArticleId><ArticleId IdType="doi">10.1016/j.bios.2014.01.026</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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